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KMID : 1102020180480010006
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Applied Microscopy
2018 Volume.48 No. 1 p.6 ~ p.10
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Workflw of Cryo-Electron Microscopy and Status of Domestic Infrastructure
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Choi Ki-Ju
Shin Jae-In
Lee Sung-Hun
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Abstract
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Cryo-electron microscopy (cryo-EM) allows the analysis of the near-native structures of samples such as proteins, viruses, and sub-cellular organelles at the sub-nano scale. With the recent development of analytical methods, this technique has achieved remarkable results. The importance of cryo-EM gained wide recognition due to last year’s award of the Nobel Prize in Chemistry. To help promote the knowledge of this technique, this paper introduces the basic work?ows of cryo-EM and domestic cryo-EM service institutes.
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KEYWORD
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Cryo-electron microscopy, Workflw, Single particle analysis, Cryo-electron tomography
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